Microfluidics Workshop

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The Lurie Nanofabrication Facility (LNF), in association with the Microfluidics Biomedical Sciences Training Program (MBSTP), will be hosting a workshop on microfluidics March 6th. The workshop will consist of classroom seminars, guest lectures, and hands-on experiences. While all talks are free, there will be a fee for the hands-on part of the workshop.  Advanced registration will be required for the hands-on experience.

The workshop is open to everyone and no fabrication experience is required. Space is limited, so register early!

Event: LNF Microfluidics Workshop

Date: March 6th, 2014

Time: 8:00 am – 5:30 pm

Lecture Location: Room 1670, Bob and Betty Beyster Building (BBBB), 2260 Hayward St. Ann Arbor, MI 48109

Topics Include:   

  • Microfluidics, BioMEMS
  • Controlling microflows (laminar vs. turbulent)
  • Computer simulation and modeling of microfluidic systems
  • Leveraging next-generation capabilities at the LNF for your design

Schedule:

8am:            Registration

8:30am:      Introduction to Microfluidics: Design and Fabrication

10am:         Dr. Rohit Karnik, Associate Professor at MIT and leader of the Microfluidics and Nanofluidics Research Laboratory  (MBSTP seminar*)

Talk Title: Direct Separation and Analysis of Cells Mediated by Transient Molecular Interactions in Microfluidic Devices

Abstract: Multiple sample-processing steps present a challenge for the development of low-complexity devices for laboratory or point-of-care separation and analysis of cells. In this talk, I will discuss a new approach that can directly separate, enrich, or analyze cells with minimal or no sample processing requirements. We show that transient cell-surface adhesive molecular interactions can exert forces on the cells that can direct the trajectories of cells flowing through microfluidic devices. Such interactions occur in cell rolling, a physiological phenomenon involved in cell trafficking where transient molecular bonds are continuously formed and broken as the cell rolls on a surface under the action of hydrodynamic forces. Using this approach, we demonstrate separation of cells with high purity and efficiency in parallel microchannel devices, and direct separation of neutrophils from blood with ultrahigh enrichment in a neutrophil activation-dependent manner. We extend this approach to controllably contact mesenchymal stem cells with receptor-coated surfaces to quantify cell adhesion behavior by visualization of their trajectories in a “cell adhesion cytometer”, which can track changes in the cell phenotype. The results demonstrate the potential of the emerging technology of using transient cell-surface molecular interactions to directly separate and analyze cells for point-of-care diagnostics, isolation of rare cells, quality control of stem cells, and other applications.

Bio: Rohit Karnik is Associate Professor of Mechanical Engineering at the Massachusetts Institute of Technology, where he leads the Microfluidics and Nanofluidics Research Group. He obtained his B. Tech. degree from the Indian Institute of Technology at Bombay in 2002, and his PhD from the University of California at Berkeley in 2006 under the guidance of Prof. Arun Majumdar. After postdoctoral work with Prof. Robert Langer at MIT, he joined the Department of Mechanical Engineering at MIT in 2007. His research focuses on the physics of micro- and nanofluidic flows and design of micro- and nanofluidic devices for applications in healthcare, energy systems, and biochemical separation and analysis. Among other honors, he is a recipient of the Institute Silver Medal (IIT Bombay, 2002), NSF Career Award (2010), Keenan Award for Innovation in Undergraduate Education (2011), and DOE Early Career Award (2012).

11am:         Dr. Kalyan Handique, co-founder of HandyLab, Inc., and now CEO of DeNovo Sciences LLC                               (MBSTP seminar*)

Talk Title: Lessons learnt from commercialization of microfluidic devices

Abstract:  This talk will go through the entrepreneurial journey of HandyLab, a University of Michigan molecular diagnostics start-up, from its inception in 2000 to its eventual sale to Becton Dickinson in 2009. The talk will focus on the experience of developing and commercializing HandyLab’s microfluidics technology, securing funding from various sources including venture capital, building a world-class team, developing and selling a molecular diagnostic product platform in a regulated medical market, and eventual exiting through the sale of HandyLab to BD for $275MM. Key lessons learnt related to the commercialization of the microfluidic technology as well as building the Company will be discussed. Dr. Handique will also touch upon how he applied some of the lessons learnt earlier to the new cancer diagnostics start-up called DeNovo Sciences.

Bio: Kalyan (Handy) Handique is currently the President and CEO of DeNovo Sciences, the Grand Prize winner of 2011 Accelerate Michigan. DeNovo Sciences is developing a microfluidic platform for detection of cancer from blood as an alternative to painful biopsies. Prior to joining DeNovo, he was VP of R&D Systems Development at Becton Dickinson (BD) Diagnostic Systems. He became a part of BD as result of a landmark deal valued at above $275 Million, where BD acquired Handylab, a company he co-founded in 2000. Handy played a pivotal role in forming the vision, the core technical team as well as envisioning the positive, vibrant culture of HandyLab, a molecular diagnostic company. He started Handylab while completing his doctoral degree under Prof Mark Burns, Chemical Engineering at University of Michigan. Under his leadership, the highly motivated HandyLab team successfully launched a series of integrated molecular diagnostic products in the hospital testing market. Handy is the author of numerous journal articles, such as in Science, as well as the inventor of over 50 patents and patent applications.

12pm:        Lunch and networking

1pm:           Hands-on workshop. The objecive of the workshop is to manufacture a microfluidic device that mixes fluids, shows the different fluid flow regimes (laminar and turbulent) and different fabrication details and applications.  There will be 5 stations all running 45 minutes long.

Station 1: Fabrication of SU-8 mold.  This module will involving spin-coating, curing, exposure details and development.

Station 2: Handling PDMS.  This module will involve various tools and techniques for mixing and pour PDMS.

Station 3: Device assembly.  Post processing of the PDMS mold and bonding.

Station 4: Metrology. Using various tools and capabilities to measure and gauge our microfluidics device.

Station 5: Modeling.  Designing and simulating the device and its functionality.

* Registration for MBSTP seminar is encouraged.

Hands-On Registration Fee:

  • General public: $250*
  • Academic researchers/ students: $150*

*Of which $50 is a credit towards LNF user fees if the attendee subsequently becomes a user of the LNF.

Click the button below to register for this event.

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If you have any questions and/or need more information, please contact: Dr. Khaled Mnaymneh at kmnay@lnf.umich.edu.

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